Journal: International Microbiology

Article Title: Is reduced ferredoxin the physiological electron donor for MetVF-type methylenetetrahydrofolate reductases in acetogenesis? A hypothesis

doi: 10.1007/s10123-021-00190-0

Figure Lengend Snippet: Purification of the MTHFR from C. ljungdahlii

Article Snippet: 50 ml of cytoplasm (1290 mg) was applied to fast protein liquid chromatography on Q-sepharose high-performance (GE Healthcare, Chicago, IL, USA) previously equilibrated with buffer A (50 mM Tris–HCl, 20 mM MgSO 4 , 20% glycerol, 2 mM DTE, 4 µM resazurin, pH 7.6) at a flow rate of 2 ml/min.

Techniques: Purification, Activity Assay

Journal: International Microbiology

Article Title: Is reduced ferredoxin the physiological electron donor for MetVF-type methylenetetrahydrofolate reductases in acetogenesis? A hypothesis

doi: 10.1007/s10123-021-00190-0

Figure Lengend Snippet: Purification of the MetVF-type MTHFR from C. ljungdahlii. The MTHFR from C. lungdahlii was purified in 3 chromatographic steps. 10 µg protein of each step was separated in a denaturating SDS-PAGE and stained with Coomassie brilliant blue. 1, cytoplasm; 2, Q-sepharose; 3, Phenyl-sepharose; 4, Superdex® 200 Increase

Article Snippet: 50 ml of cytoplasm (1290 mg) was applied to fast protein liquid chromatography on Q-sepharose high-performance (GE Healthcare, Chicago, IL, USA) previously equilibrated with buffer A (50 mM Tris–HCl, 20 mM MgSO 4 , 20% glycerol, 2 mM DTE, 4 µM resazurin, pH 7.6) at a flow rate of 2 ml/min.

Techniques: Purification, SDS Page, Staining